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The signal peptide is removed during translation, generating proinsulin.
Western blot analysis of BCOR protein from the eyes of these mice and wild type controls revealed that an alternative start codon, 3' to the engineered frame shift, was used for translation, generating BCOR protein with a predicted 71 amino acid amino-terminal deletion (Figure 1C).
Selective use of exons results in a frame shift in translation, generating a WW-containing motif at the C-terminus of FHL1C, which can bind to RBP-J.
Within hours, a later phase of mast cell activation through IgE becomes evident with the induction of new gene transcription and translation, generating a host of cytokines and chemokines (Table 1).
Although the pioneer round of translation was initially believed only to have a quality control role in orchestrating NMD (nonsense-mediated decay) [ 129, 130], evidence suggests that CBC-bound transcripts can undergo multiple rounds of translation generating functional products [ 131– 131].
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Because nick translation generates many labeled fragments of random length, we concluded that this method disrupted secondary structure that otherwise prevented the amplicons from hybridizing to their respective probes.
The ∼9 kb genome contains two non-overlapping open reading frames (ORFs) whose translation generate separate polyproteins that are proteolytically processed into several non-structural and at least three structural proteins.
First, the inherent stochasticity in biochemical processes such as binding, transcription, and translation generates the intrinsic noise due to random encountering, whose relative magnitude is proportional to the inverse of the system size.
TEV genome is composed of a 9.5 kb positive polarity single-strand RNA that encodes a large ORF whose translation generates a polyprotein that is subsequently self-processed by virus-encoded proteases into 10 mature peptides [19], [20].
Altogether, the 15 TLR5 SNPs that we detected in our cohort were grouped in 14 alleles whose translation generates 12 forms of the TLR5 protein (Table S4) among which those containing the new non-synonymous changes alone occur at low frequency (<1%).
The observation that RAN translation generates dipeptides in C9ORF72 mutant cases is of great interest (Mori et al., 2013b).
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