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The nucleotide subject data within a window is obtained and translated using that window's translation frame.
A construct in the correct translation frame was used to transform BL21 cells and express recombinant AgApoLp-III protein.
Biologically, a shift in the start or end position of a coding exon, caused for example by an insertion or by inaccurate splicing, can have dramatic consequences, possibly causing a translation frame shift.
The contigs were annotated taking into consideration the identity of the translation frame, the conserved protein domains and Gene Ontology (GO) terms.
We identified the sequence that aligned with the three protein sequence sets with the same translation frame as the appropriate sequence for the microarray probe design.
Therefore, in accordance with this observation and with respect to the translation frame of LDLR exons, protein variants could be predicted (Table 1).
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We also observed differential usage of distinct 5'- and 3'-UTRs as well as alternative translation frames in the amino- and carboxy-terminal coding regions.
The numeral (0, 1, 2) appended to the unitranscript sequence identifiers indicates the translation frames of the putative MYB, with ".0" indicating the +1 frame, etc.
First, each sequence stored in a nucleotide database may contain more than one coding region in the same or different translation frames.
The presence of conserved domains was researched and annotated using InterProScan [ 70] on the six possible translation frames of each contig.
Each nucleotide dataset was scanned (in each of the six possible translation frames) with the HMM for the PEP-CTERM domain (TIGR02595).
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