Exact(26)
Results from expression studies using trpE′-′lacZ translational fusions and RNA-directed cell-free translation experiments suggest that the Mg2+-dependent tertiary structure inhibits TRAP's ability to regulate translation of trpE.
The in vitro translation experiments (Fig 4) showed evidence for translational stalling at up to 14 specific positions.
We then delivered our single molecule translation mix (as described in the section below) and allowed RNCs to translate for 20 min at room temperature, which based on our active translation experiments, is enough time to ensure full translation of the mRNA.
Researchers have also benefited from a much faster means of evaluating the outcome of translation experiments: a computerized technique developed by I.B.M. enables researchers to test 10 to 100 new approaches for cracking languages each day.
Translation experiments have been performed from Spanish to English and from Italian to English in an application involving the interaction of a customer with a receptionist at the frontdesk of a hotel.
The purified mRNAs were used for in vitro translation experiments.
Similar(34)
This data is consistent with the in vitro translation experiment, confirming that the translation of HIC is repressed by the HIC mRNA 5'-UTR in transfected cells.
In contrast, an all-enzymatic system using the wild-type AARSs would be as convenient as a standard translation experiment using the twenty proteinogenic amino acids.
Using the plasmid pMPF2488 as template in an in vitro translation experiment was also found to yield a protein band of the expected size (54 kD).
Morpholinos were tested for the ability to specifically diminish X. laevis hipk1 by co-translation experiments using an in vitro reticulocyte lysate transcription and translation assay (Invitrogen).
In vitro transcription-translation experiments suggested that this is largely due to alternative initiation of translation [ 11].
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