Alternatively, it has been also suggested to use translational enhancers to improve translation efficiency of the parthenocarpic gene of interest [ 20].
The first two groups of experiments measure the translation efficiency of POS.
Therefore, the translation efficiency of different proteins through the identical RBS sequence of R3 or R9 may be dramatically different.
The translation efficiency of GadB2 was improved in R4a-B2, where gadB2 was expressed by P tacM promoter.
Using our training data, we developed a methodology to predict and improve the translation efficiency of open reading frames (ORFs).
However, in such future studies, we suggest taking into consideration the possibility that the translation efficiency of Ct-OATP1B3-v1 or Ct-OATP1B3-C might be very low.
We find that the translation efficiency of endogenous genes is regulated by unfolding kinetics of structures overlapping the ribosome binding site.
Short uORFs in the 5'-UTR affect translation efficiency of many eukaryotic genes [16] [22].
It is possible that translation efficiency of elevated Cdx2 mRNA is negatively controlled by unknown mechanisms, such as micro RNAs.
The translation efficiency of a control mRNA encoding for the EF1A translation factor was evaluated in the presence of 200 µM of the indicated compounds (Figure 4c).
Mutations in uORF1 or uORF2 may probably affect the translation efficiency of the following uORF, but not the translation of the main ORF.
