In this regard, rolA mRNA appears to be poorly translated in a wheat germ translation assay [ 18, 19].
Finally, we tested the influence of gene optimization on translational efficiency using a cell-based translation assay.
(A ) Read density plot showing phasing of ribosome footprinting reads in the frame of smORFs within CR30055 and pncr009 3L detected as translated and confirmed by FLAG immunofluorescence translation assay.
The observation that PYM directly enhances the translation of gM in the in vitro translation assay is, however, not unexpected, as this assay identifies general translational enhancers, a category occupied by PYM due to its ability to stabilize ribosomes (Diem et al, 2007).
In our hands, water was the best extraction agent for mycotoxin when using this specific luminescence protein translation assay kit.
The suspensions were used immediately in the in vitro luminescence protein translation assay with the trichothecene, T-2 toxin, as a control.
A similar inhibitory effect was observed when a specific peptide based on the exposed region was used in the cell-free protein translation assay.
In order to form a complex with LFn-GAL4, ASOs were engineered to contain a double-stranded region, and a cell free in vitro translation assay demonstrated that no loss of antisense activity above 30 pmol ASO was evident.
The experiments were designed to specifically evaluate a novel method of mycotoxin extraction, solubilization, and the subsequent inhibitory effect in an established in vitro luminescence protein translation assay from 30 day-old spores.
Based on a cell-free coupled translation assay the actin variants could be graded by their tendency to associate with the chaperonin TCP-1 ring complex/chaperonin containing TCP-1 (TRiC/CCT) as well as their propensity to acquire their native conformation.
