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After translating the sequence corresponding to one gene, the same translational machinery could continue and directly start the translation of the following gene [ 11, 12].
In silico validation of this assembled transcript is achieved by translating the sequence (http://web.expasy.org/translate) and by searching protein databases using the Protein Basic Local Alignment Search Tool Standard Protein BLASTT, http://blast.ncbi.nlm.nih.gov; Figure S4B).
The transcriptome analysis is an important tool to help identifying putative function of genes, translating the sequence of nucleotides in a sequence of amino acids, which is more likely to be conserved [ 20, 21].
This shows the applicability of ILP marker-based comparative genome mapping between foxtail millet and other grass species such as sorghum, maize, rice and Brachypodium in translating the sequence information/candidate genes from this diploid crop to other polyploid biofuel grasses.
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We also evaluated the potential protein coding capacity of sRNAs by translating the sequences in all three open reading frames.
In the third step, we directly use the phase to translate the sequence for the exons with the phase information in Ensemble transcript.
We first translate the sequence profile of the candidate solenoid repeat into the numerical functions derived by Atchley and collaborators (Atchley et al., 2005).
Chromas, version 1.5, software (Technelysium, South Brisbane, Australia) and Lasergene package computer programs (DNASTAR, Madison, Wisconsin) were used to edit, assemble, and translate the sequence.
We have translated the sequences to amino acid space and this set of genes forms our reference kinome aref.
Therefore, we implemented a second approach in which we translated the Sanger sequence of each patient into position-wise probabilities for A, C, G and T. We then translated the sequences into amino acid sequences with corresponding position-wise probabilities.
Putative protein sequences were obtained by translating the cDNA sequences using the EMBOSS program "Transeq" [ 57].
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