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Both write requests (which involve translating in-memory objects or API calls to native API calls) and read requests or search queries (which involve translating database objects to application objects) rely extensively on database mapping.
TBLASTN operates by translating database nucleotide sequences to hypothetical amino acid sequences in all six reading frames and then aligning the hypothetical amino acid sequences to the query.
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A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database.
Database searching was performed by first generating the full 6-frame translated database and an equivalent reverse decoy database [36].
In some previous researches, a filter of protein length is applied to the translated database.
P (I k | F, X > x ) depends on the sizes of the annotated database and the translated database.
The BLAT manual suggests that for mapping ESTs to a genome across species one use a translated query against a translated database (- q = dnax and - t = dnax).
This probe was used to isolate several cereal Type I-like MADS-box sequences from available EST databases and the GenBank non-redundant CDS translated database.
We searched the genomic zebrafish (Danio rerio) database (see Methods) with the amino acid sequence of TnPKR1 in order to identify a PKR gene using protein query versus translated database (tblastn).
Tribolium CDS were downloaded from ftp://ftp.bioinformatics.ksu.edu/pub/BeetleBase/ (version 3) and mapped to the X. vesparum genome using blat with a translated database and query and only the best hit was kept.
Genes of all three species were therefore first mapped to the A. pisum gene sequences using BLAT with a translated database and query (Kent 2002), filtered for a minimum score of 50.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com