The level of translated protein from 756 expression constructs was measured and the influence of a large number of possible effector attributes was statistically analyzed.
A possible effect of a functional variant beyond changes in amino-acid composition of translated protein could lead to altered gene expression, which can be modulated by the concerted action of multiple transcription factors over long distances and, thus, be influenced by multiple SNPs [40].
A number of clones were selected in this manner over the course of 1 2 weeks (post-transfection) and then assayed for expression of the translated protein using western blots.
Expression of mRNA does not necessarily mean that there is translation into protein or that translated protein is functional, but nevertheless provides a powerful tool for initial screening.
Each step of this journey impacts the ultimate expression level and functional capacity of the translated protein.
In order to expand on these results and further evaluate if the effects at the mRNA level translated to protein expression, we selectively depleted the p65 subunit in U2OS cells using siRNA and then evaluated the effects of doxorubicin treatment on anti-apoptotic proteins.
Our findings also suggest expression of myofibroblastic marker mRNA translated to protein expression in early callus fibrous regions.
Data from gene expression profiling experiments are describing transcriptional changes of mRNA and cannot be necessarily be translated into protein expression.
In this proof-of-concept study, we used a novel high-throughput system, using affinity-purified, mono-specific antibodies, to translate protein targets from gene expression studies into clinically applicable IHC-based prognostic panels for breast cancer.
Further studies are needed to clarify how abundant TSPO expression is in our dedifferentiated melanocytes and how it translates to protein expression.
