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The lower sequence is the amino acid sequence of the fragment translated in the frame that resembles a GH7 family protein.
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In summary, all TGA-containing ORFs, defined as genomic sequences between two non-TGA stop codons with at least one in-frame TGA and no other in-frame stop codons, are searched in the genome of interest; the resulting sequences are translated in the appropriate frame and compared against the non-redundant protein and EST databases, as well as against other genomes (of insects, in this case).
The entire sequence of each paralog was translated in the reading frame given by the tBLASTx search.
EST or cDNA sequences thus selected are translated in the reading frame corresponding to the query sequence using Transeq (17).
Selected EST sequences are translated in the reading frame corresponding to the query sequence using Transeq (17).
In order to identify any amino acid substitution that may have functional implications between different virus haplotypes, the sequences from each marker were aligned to the corresponding gene sequence downloaded from NCBI GenBank, then translated in the corresponding frame using the standard genetic code.
However these are similar to repeat regions present in the gene [ 98] and can be translated in-frame with the coding exon, leading to the conclusion that all KRTAP are intron-less [ 14, 99].
Each transcript was translated in the 3 frames and checked against the PRIDE peptides.
The T. thermophila matching sequences were retrieved and translated in the 6 frames.
However, if the knockout targeting construct does not include the relevant protein region to which the antibody was raised, it is possible that the antibody could react to a protein translated in-frame from the targeting construct in vivo, and lack of staining is not a foregone conclusion.
All of the introns could be included in a primary transcript that can be translated in-frame with the coding exons, they just have longer repetitive regions.
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