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Predicted amino acid sequences were generated using the DNA sequence translate tools EXPASY http://www.expasy.ch/tools/dna.html.html
To annotate the Rabs in these contigs, the DNA sequence of these contigs was put in the translate tools of Expasy.
To annotate the β-subunit of GGTase-II in Leptomonas, the DNA sequence was retrieved from the contig (genes_5507) of its genome and was put in the translate tools of Expasy.
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In silico translation (Expasy, translate tool [ 46]) and gene structure prediction (GENSCAN Web Server http://genes.mit.edu/GENSCAN.html, [ 47]) were performed on the opsin-like sequences retrieved from the draft genome of A. filiformis.
Open reading frames were located with ORF finder (http://www.ncbi.nlm.nih.gov/gorf/), and translation was carried out using the ExPASy translate tool (http://web.expasy.org/translate/).org/translate/
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Each sequence was also translated using the translate tool in ExPASy (http://www.expasy.org/).org/
Nucleotide sequences were translated using the translate tool in ExPASy Bioinformatics Resource Portal.
Potential open reading frames (ORFs) were searched using the Expasy translate tool [ 75].
ExPasy "translate" tool was used to identify open reading frames (ORFs) in these unigenes [ 53].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com