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The general approach has been to translate sequence data to get in silico maps and compare these to experimentally obtained maps using dynamic programming algorithms.
Another possible explanation is that the relative occupancy measurements made by techniques such as ChIP are not the most relevant parameter by which TFs translate sequence into expression output.
The challenge now will be to work out how to translate sequence information into chemical reality, and further, how to begin to access not just small variations in old chemistry, but completely new chemical scaffolds.
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Chromas 1.5 software (Technelysium, USA) and LASERGENE package computer programmes (DNASTAR, USA) were used to edit, assemble, and translate sequences.
(10) 3' UTR exons contained 3' non-translated exons or their parts, excluding overlaps with any translated sequence.
The translated sequence is similar to that of chymopapain isoform II (73%) and CC-III (77%) from C. candamarcensis.
Partial sequencing of the PCR products gave a translated sequence 100% identical with the amino acid sequence of P. aeruginosa amidase.
The DNA fragment was translated to the corresponding polypeptide in silico and a BLASTX was performed to compare the translated sequence with alkaline protease precursor protein.
The translated sequence of this DNA fragment showed a certain degree of similarity to the amino acid sequence of RNA-dependent RNA polymerases from other dsRNA viruses.
The translated sequence contained 203 amino acids (molecular mass of 22,064.63 Da) with characteristic lysine rich regions and a pI=pH 10.75.
The translated sequence of 115 amino acids possesses a molecular mass of 12,681.4, a pI of 6.27 and a stability index of 21.48.
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