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The experimentally measured kinetic parameters and equilibrium transition profiles suggest a highly specific two-state transition in which the folding and unfolding of the first G3T monomer is rate-limiting for both annealing and melting processes.
To assess the thermodynamic characteristics, the quality and the purity of the molecular beacon, a melting curve analysis was performed by monitoring the fluorescence thermal transition profiles using the 7900HT Real-Time PCR System (Applied Biosystems, Foster City, California, USA).
RMA-normalized intensities were then converted into discrete transition profiles.
However, we obtained only 37 different transition profiles, 24 of which contained at least ten genes.
A clear difference between the transition profiles of a native protein structure and decoy/modelled structures is visible.
In total, the 3 = 9 diploid profiles combined with the 3 haploid profiles can form 81 possible transition profiles.
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In this study, two different types of external transition profile were considered in order to use lower ultrasonic-generator power for preventing pad damage.
The results of theoretical analysis concluded that the various parameters such as heat capacity curve, transition profile, half widths and sharpness of the transition are in good agreement with the experimental measurements for binding of thionine determined through DSC.
No imputation was done for the Rotterdam Transition Profile where questions were unanswered.
Each gene is thereby characterized by a discrete transition profile denoted by a string of the letters u, d and s.
Figure 1 shows the transition profile of 7 randomly selected native protein structures and their corresponding 981 model structures.
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