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On histology, there was no difference between groups, despite previous reports that the dnLRP5 osteosarcoma cells specifically undergo a mesenchymal-to-epithelial transition in vitro.
Importantly, iPSC-CMs cultured on 4%PEG-96%PCL demonstrate troponin I (TnI) isoform switch from the fetal slow skeletal TnI (ssTnI) to the postnatal cardiac TnI (cTnI), the first report of such transition in vitro.
In contrast, RNAi-mediated knockdown of TBLR1 inhibited epithelial mesenchymal transition in vitro and in vivo.
Recently, it was shown that the purified Nck and N-WASP proteins can undergo a phase transition in vitro.
The second, Tfe3 relocalises from the nucleus to the cytoplasm during the implantation transition in vitro and in vivo.
GLA induced the mesenchymal epithelial transition in vitro and in vivo, as determined by increased expression of the epithelial marker, E-cadherin, and decreased expression of the mesenchymal marker, vimentin.
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We use an approach involving a quantitative model of cell-state transitions in vitro to gain insights into how SLUG/SNAI2, a key developmental transcription factor, modulates mammary epithelial stem cell activity and differentiation in vivo.
Xenopus egg extract supports all the major cell-cycle transitions in vitro.
To test whether Edc3 and Pdc1 are able to induce phase transitions in vitro we initially tried to purify the Pdc1 protein from E. coli.
We then used this designed dimeric Pdc1 protein (that has an HLM valency of six) and the dimeric Edc3 protein (that has an LSm valency of two) to test whether Pdc1 and Edc3 can undergo phase transitions in vitro.
Herein, we use a bottom-up approach that combines NMR spectroscopy, isothermal titration calorimetry, X-ray crystallography, and fluorescence microscopy to probe if mRNA degradation factors can undergo phase transitions in vitro.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com