Detected p53 mutations were mainly GC to AT transitions at CpG sites.
Of the 26 mutations recovered, nearly all (92%) were GC to AT transitions at CpG sites, supporting previous data with regard to p53 mutations in Barrett's tumours (Gleeson et al, 1995, 1998).
However, novel patterns of p53 abnormalities, including unique sites or types of mutation (i.e. an excessive proportion of G C to A T transition at CpG site), and accumulation of wild-type p53 either within nuclear or cytoplasmic compartments were noted.
Positions were classified as synonymous based on the following criterion: The consensus amino acid at this position did not change throughout infection and at least the transition at this site is synonymous.
The processes required for metastasis of epithelial cells from primary tumors closely resembles that of cells undergoing EMT followed by mesenchymal to epithelial transition at the site of arrest [ 1- 3].
This work was aimed at recovering and dating plant and animal remains that would illuminate the nature of the Mesolithic-Neolithic transition at these sites.
Notably, the rate of CpG mutations varied according to TE order and superfamily examined: TE copies exhibiting a pattern of frequent transition at CpG sites appeared lower for class II DNA transposons (Helitron-type and TIR elements) than that of class I Retrotransposons (21%and58%58 % respectively) (Additional file 10).
The resulting I-T mismatch in DNA is potentially mutagenic since a dI in the DNA template prefers to pair with dCTP during replication, yielding A T to G C transition mutations at sites of adenine deamination [ 5].
Deoxyinosine derived from deamination of deoxyadenosine in DNA is potentially mutagenic since it prefers to pair with dCTP during replication, yielding A T to G C transition mutations at sites of adenine deamination [ 5].
