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Both cDNAs carry a putative transit sequence for chloroplast import and differ mainly in their length and the 5′-flanking regions.
This protein was the first protein demonstrated to be addressed to the inner membrane of the chloroplast envelope while lacking a classical and cleavable transit sequence [16].
In Narcissus, the full-length PSY enzyme showed reduced catalytic activity when compared with an N-truncated form with its transit sequence of 214 bp removed by site-directed mutagenesis [29].
RLSB contains a plastid transit sequence, and co-localizes with LSU to chloroplasts.
The Glyma20g24766.1 transcript appears to contain a chloroplast transit sequence as predicted using ChloroP1.1, Technical University of Denmark, http://www.cbs.dtu.dk/services/ChloroP/, while Glyma20g24766.2 does not contain this 5′ transit sequence.
For example, only one of four predicted AOS genes in rice carries a putative chloroplast transit sequence [ 59].
Similar(41)
Interestingly, the genes with transit sequences have trans-eQTL that map within 200 kb of Tomm40l, suggesting that this gene might in fact be causal for the eQTL mapping to this sub-region.
When compared to the original primary sequence deduced from the corresponding cDNAs, production of the mature forms of these proteins thus required the deletion of the first 60, 79 and 102 amino acid residues corresponding respectively to the chloroplast transit sequences of AtHMA1 [22], AtAATP1 [28], [28] and AtHMA6 [21].
In contrast, GSIIB proteins of P. patens lacked transit sequences, suggesting a cytosolic function.
Anyway, the repertoire for creating novel targeting sequences or for replacing the transit sequences from the primary host cell by bi- or tripartite presequences did already exist.
Both GSIIB proteins in C. reinhardtii and V. carteri f. nagariensis had N-terminal transit sequences, indicating they are targeted to the chloroplast or mitochondrion.
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