This property also distinguishes persistence from heteroresistance, a phenomenon in which a small subpopulation transiently displays a substantially (more than eightfold) higher MIC14 (see Box 1).
Although SCVs transiently display markers characteristic of early endosomes, e.g., Rab5 and transferrin receptor, they rapidly uncouple from the default endocytic pathway so that interaction with host lysosomes is limited and bacterial degradation is evaded (Garvis et al., 2001; Steele-Mortimer et al., 1999).
Cybrids transiently overexpressing Cterm displayed increased cell viability after 48 h of incubation in GPM (up to 4 fold as compared to mock transformants) and decreased apoptosis after 24 h (up to 3.5 fold decrease as compared to mock transformants) (Fig 3A, B and supplementary Fig S5).
The remaining patients had transiently detectable levels that displayed no discernible pattern, and no patient had a level exceeding 20 ng/ml.
STB5 expression level displayed transient induction in both Mf-fruit and Mf-Pdig interactions at 24 h, while the Ser-Thr kinase was transiently repressed in both Mf-fruit and Mf-Pdig interactions at 24 h.
U87 or LN-18 GBM cell lines, transiently transfected with miR-125a/b precursors, displayed significantly lower luciferase activity for reporter constructs containing the binding site for TNFAIP3 or NKIRAS2 relative to the control construct containing no binding site.
Of note, L22 proteins expressed transiently in 293T and HeLa cells typically displayed abundant nucleolar but little cytoplasmic fluorescence, whereas stably expressed GFP-L22 was observed in nucleoli and in the cytoplasm (Fig. 5A).
Moreover, the equivalent bBest2 I201T mutant was not stimulated by 10 mM ATP in transiently transfected HEK293 cells (Supplementary Figure 3c), and displayed no affinity to ATPγS in MST (Supplementary Figure 3d).
The two remaining animals displayed minimal clinical signs of arthritis transiently and received a subcutaneous booster immunization with bo-CII in IFA at day 114.
