Intermediate progenitor cells (IPCs) are transient transit-amplifying progenitors present during neocortical neurogenesis that arise by asymmetric divisions of radial glial progenitors (Haubensak et al. 2004; Miyata et al. 2004; Noctor et al. 2004).
The expansion of stem cell pool supports a transient expansion of transit amplifying population up to 14.5% (equivalent to one to two cells; Figure 5).
Results of tissue restitution following 50% reduction in differentiated epithelium are shown in Figure 3B, where the epithelium renewed 35% faster than normal and the pool of stem cells expanded from 4.1 to 8.1 per section of crypt (Figure 4B), accompanied by a transient expansion of transit amplifying population up to 14.7% (equivalent to one to two cells; Figure 5C).
Results are shown in in Figure 3B, where the epithelium renewed 40% faster than normal and the stem cells expanded from 4.3 to 8.6 per section of crypt (Figure 4C), accompanied by a transient expansion of transit amplifying population up to 11% (equivalent to one cell; Figure 5C).
However, through various pharmacological treatments and molecular methods it was determined in SH-SY5Y cells that the presence of GSK3β in the nucleus is transient [22], and its transit between the cytosol and the nucleus is highly dynamic [49].
Transient transfections were performed with TransIT-LT-1 transfection reagent (Mirus, Madison, USA).
Feedback loops of this type are exactly what is required to generate a transient wave of destabilised chromatin during transit of Poll II.
Transient transfections were performed by using TransIT-LT-1 transfection reagent (Mirus, USA) according to the manufacturer's instruction.
Transient plasmid transfections were performed using TransIT-LT1 (MIRUS, Madison, WI) or Lipofectamine 2000 (Invitrogen, Carlsbad, CA) reagents, according to the manufacturer's protocols.
Retro- and lentiviral supernatants were generated by transient transfection of 293T cells following the TransIT transfection protocol (Mirus Bio, Madison, WI) and harvested 48 hr later.
Under equilibrium conditions, it is possible that τD2 represents the extent of transient ligand dissociation or, alternatively, association during transit of the receptor through the confocal volume (Fig. 9 B).
