Sentence examples for transient transformations from inspiring English sources

For lily and tobacco pollen, we performed transient transformations with the same fusion construct under the control of the zmC13 and Lat52 promoters, respectively [34], [35].

Note also that epifluorescence images All fluorescence images of cells shown in the figures are representative of >50 independent (transient) transformations from at least two independent transformation experiments.

When using the agroinfiltration technique in transient transformations in tobacco, the mRNA of Zera fusions usually decayed after 7 dpi [25].

Transient transformations, including those involving Arabidopsis leaf epidermal cells (Figure S2c), were performed using 10 µg of plasmid DNA (or, with one exception [see below], 5 µg of each plasmid for co-transformations) with a biolistic particle delivery system Bio-Rad Laboratories Ltd.., Mississauga, Canada) [44].

ILT performed transient transformations in plants and confocal microscopy.

CTH carried out the majority of the experiments, including plasmid constructions and transient transformations.

The promoter-LUC fusions were used in transient transformation by mixing 100 μl of Agrobacterium strain GV3101 (MP90) transformed with the reporter cassette with or without another Agrobacterium culture(s) (450 μl) transformed with a cassette containing MYB10, bHLH3 and/or MYBPA1 fused to the 35S promoter.

The cassettes were then cloned into pBIN19 with HindIII/EcoRI and CPK3 was exchanged for other genes using ApaI and NotI. A. tumefaciens (AGL1 strain) was transformed with the resulting plasmids by electroporation and further used for transient transformation of tobacco leaf epidermis.

AKIN10 was amplified from cDNA by PCR (see Table 1 for primers) and cloned ApaI/NotI into pBIN19 containing a c-terminal mCherry tag. A. tumefaciens (AGL1 strain) was transformed with the resulting plasmids by electroporation and further used for transient transformation of tobacco (N. tabacum) leaf epidermis as described above.

Although a transient transformation of T. borchii mycelium has already been obtained [ 35], stable transformants cannot be exploited to provide a direct functional evidence to support this hypothesis.

Protoplast transient transformation was used to observe the locations of IiPALs.