Although a transient transformation of T. borchii mycelium has already been obtained [ 35], stable transformants cannot be exploited to provide a direct functional evidence to support this hypothesis.
Protoplast transient transformation was used to observe the locations of IiPALs.
Transient transformation and gene silencing was performed by Endo et al. ([2008]) in Zinnia elegans cell cultures differentiating TEs.
Fig. 8 Subcellular localization of REL2. a Analysis of the subcellular localization of REL2 using a tobacco transient transformation system in the cells of tobacco leaves.
For subcellular localization analysis in tobacco leaf epidermal cells, Agrobacterium tumefaciens mediated transient transformation of N. benthamiana was performed as described (Li et al. 2008; Voinnet et al. 2003).
Meanwhile, the set process is characterized by transient transformation, and the reset process is a gradual oxidation procedure, in which oxygen ions are propelled to rupture the conduction filament.
There is evidence that transient transformation of tobacco leaves is a very efficient system for producing rapid and scalable high rates of recombinant proteins [37].
In planta transient transformation assays were performed in young Arabidopsis seedlings and fluorescence was recorded 3 days later by confocal microscopy [127].
Nicotiana spp have served as a host for the heterologous production of many medically relevant proteins using nuclear, chloroplast and transient transformation approaches (reviewed by [11]) and also for the production of amorphadiene.
GR lip was used for transient transformation.
Wild-type Nicotiana benthamiana plants were used for transient transformation.
