Sentence examples similar to transient suspension from inspiring English sources
For transient transfections, suspension CHO-DG44 cells were seeded at a density of 3 × 10 cells/ml into CD DG44 Medium Life Technologiess) with 8 mM L-glutamine (Invitrogen) and 0.18% Pluronic® F-68 (Invitrogen) in a 250 ml shake flask 3 days before transfection.
Transient production in suspension HEK293-6E cells was performed as previously described [ 38].
Serum-free suspension transient transfection is thus a viable option for high-yield-rFVIII production.
The transfections were performed using Lipofectamine™ 2000 Transfection Reagent following the manufacturer's protocol for transient transfection of suspension cells.
We also investigated directly the role of surface area by transducing the same cell population (CHO K1) in optimal low pH conditions as adherent cells or following transient lifting into suspension using a non-trypsin stripping agent.
To our knowledge this is the first study describing a rFVIII production method based on transient transfection in suspension serum-free cultures that can be operated at large scale.
SA (NCXCL-SAC and NSA-scFvC) and Fc (NFc-CXCLC) fusion proteins were expressed by transient transfection of suspension-adapted human embryonic kidney (HEK293) cells.
The cDNAs were synthesised, cloned into expression vectors and protein was expressed by transient transfection of HEK suspension cells (Geneart AG, Life Technologies, Regensberg, Germany).
Transient assays in sugarcane suspension cell cultures revealed the synthetic luc* gene produced on average 60-fold higher luciferase activity relative to the native firefly luciferase gene.
Using a novel transient assay in sugarcane suspension cells combining co-bombardment and qRT-PCR, we showed that synthetic luc* and Ren luc* genes generate increased transcript levels compared to the native firefly and Renilla luciferase genes.
Here we show that small scale transient transfection of mammalian suspension cells together with analysis by dot blot can be used to assess positive expression hits in a multiparallel high throughput manner.
