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As she says, tranquillity in her life tends to be transient at best, "the calm before the storm".
After transient transfection of FLAG-FHL2 and YFP-CALM-expressing plasmids in HEK293T cells, and immunoprecipitation of YFP-CALM using GFP-binder beads, the co-precipitated FHL2 protein could be detected with anti-FLAG antibodies.
One tool for Mindsight is a "wheel of awareness," where the hub is our calm, receptive, and clear Self, and around the rim are the transient feelings, thoughts, and memories that flicker through our mind.
As the woman stands by the jewelry box and Judgment scene, her calm expression indicates a realization: she must maintain balance in her own life by not allowing transient worldly treasures to outweigh lasting spiritual concerns.
Once PtdIns4,5P2 is hydrolyzed, the avidity effect generated by a single CALM molecule simultaneously binding PtdIns4,5P2 and a small R-SNARE VAMP will vanish leaving only the weak, transient R-SNARE CALM inteR-SNARE CALM
Calm Calm Calm Nantin.
A transient.
"It was very transient".
It is transient.
"That goddamn transient company.
Evidence is transient".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com