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In GAD65, the catalytic loop (His Tyr) is mobile; this flexibility results in the transient absence of the catalytic residue Tyr from the active site.
It is widely accepted that disorders caused by EW, including increased susceptibility to diarrhea and growth retardation, mainly result from the transient absence of feed consumption that follows EW [ 1].
Occasional CGM data points were lost (8.1%), but this did not result in skipping control cycles or discontinuation of the study; by design, control to range would function during transient absence of CGM data because the controller intervenes only if risks for hypoglycemia or hyperglycemia are detected (20).
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To inform models of the [bovine chondrocyte]−[agarose gel] system, total synthesis rate (matrix accumulation rate+matrix release rate) and matrix retention fractions of glycosaminoglycans (GAG), collagen, and cartilage oligomeric matrix protein (COMP) were measured either in the presence (continuous or transient) or absence of TGF-β3 supplementation.
Presumably, such permeabilities are sustained by fractures developed at fluid pressures near lithostatic and are transient in the absence of ongoing tectonic deformation.
FtsZ polymers are normally very transient in the absence of an excess of GTP because of the high rate of GTP turnover by FtsZ (26).
For transient expressions (in absence of RNAi treatment), the cells were transfected the day after plating with 1 μg DNA per 85-mm (56 cm) dish using X-tremeGENE 9 (Roche) according to instructions provided by the manufacturer.
The inflammatory response elicited in the present study by intra-articular injection of 0.5 ng LPS was overt but also highly transient, and the absence of systemic signs of endotoxemia confirmed the local nature of LPS effects.
As predicted, both transgenes went extinct in all experimental populations within a small number of generations, indicating that transgene persistence in the field would be relatively transient in the absence of ongoing releases of additional transgenics, even from a high starting allele frequency.
The importance of the L-type Ca2+ current in generating whole-cell [Ca2+]i transients in these cells was manifested by the elimination of these transients in the absence of external Ca2+ or in the presence of nifedipine, a selective L-type Ca2+ channel antagonist.
GCaMP5K-PSD probe can detect synaptic Ca2+ transients in the absence of Mg2+ (Leitz and Kavalali, 2014 Figure 2 therein).
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