Exact(2)
The MKR mice are transgenic with a dominant-negative insulin-like growth factor-I receptor specifically targeted to the skeletal muscle with a resultant severe insulin resistance and hyperinsulinemia phenotype [ 17].
In this scenario, a mutant or transgenic with a non-lethal phenotype would be subjected to mutagenesis, and second-site mutations that were synthetic lethal with the original mutation could be identified, owing to the types of interactions outlined in Fig. 1.
Similar(58)
Usually one would use control plants with no altered protection and compare to mutants or transgenics with a well-characterised genetic alteration.
These ameliorations were intermediate in thyr-IFNγ transgenics with a single copy of LMP2 (Figure 4A right panel, and 4B fifth group).
In contrast, transgenics with a single copy of Tbr2 develop normally and do not show reduced expression (Arnold et al. 2008, 2009).
Fourteen independent T0 transgenic lines with a total of 84 transgenic plants were obtained for an E1 activity assay.
We probed their identity at the molecular level by performing immunohistochemical staining on HGn39D transgenics with an antibody against tyrosine hydroxylase, a marker of lateral-line efferent neurons [30].
The TCRα chain transgenics with an elongated CDR3 had an intermediate binding avidity.
Homozygous plants of line 78.131.1 had the highest levels of NaD1 of the pHEX22 transgenics with an average of 6.8 ppm in their leaves (Table 3).
We then compared tetramer binding characteristics of T cell lines derived from the Th2-type TCRαβ transgenic cells, TCRα chain transgenics with an elongated CDR3 and Th1-type cells from non-transgenic littermate controls.
A line was selected that expressed significant levels of the transgenic protein in the adult bone, immunohistochemically detecting the transgenic protein with a human estrogen-receptor antibody (Santa Cruz).
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