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The presence and expression of the transgene in six selected T0 transgenic wheat lines were confirmed at the molecular level.
Transgenic wheat plants were examined for the expression of the transgene by Northern blot assay.
In transgenic wheat lines, FISH analysis of metaphase chromosomes has shown that multiple transgene copies can be integrated along a single chromosome, with large regions of intervening genomic sequence.
Even as some expressed relief last week when Monsanto shelved its transgenic wheat, others formed Growers for Wheat Biotechnology to push for genetic engineering.
GmDREB1 overexpression did not activate unintended gene networks with respect to gene expression in the roots of transgenic wheat.
These findings will also facilitate the up- and down-regulation of γ-gliadin gene expression in transgenic wheat.
A significant reduction of α-amylase activity of mature grains was detected in all the transgenic wheat plants.
These experiments show that it is possible to combine biolistic transformation by minimal gene cassettes with genetic segregation to make marker-free transgenic wheat plants with new traits.
We selected drought- and salt-tolerant transgenic wheat overexpressing the transcription factor, GmDREB1, to investigate unintended pleiotropic effects using RNA-seq analysis.
Despite tremendous successes in producing fertile transgenic wheat plants using various methods and approaches, elite cultivars of wheat still remain recalcitrant to transformation.
In addition, transgenic wheat ectopically expressing a modified form of the maize Sh2 gene (Sh2r6hs) exhibits increased AGP activity in developing seeds and produces an average of 38% more seed weight per plant [66].
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