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Chimeric mice in which the bone marrow was labeled with either a transgenic marker (e.g. LacZ, eGFP) or harbored a Y-chromosome showed no contribution of cells with these labels to regenerating tubules [58].
The choice of GFP as the control is unrelated to its use as a transgenic marker for the parasite.
The common assays applied to quantify SHR rely on the precise restoration of transgenic marker genes from two incomplete but overlapping parts [9], [14].
The apparent lack of negative pleiotropic effects associated with this allele make it potentially very useful as a visible transgenic marker.
In the existing collection of FTLs there are enough XFP marker genes to track nearly every nuclear gene by virtue of its genetic linkage to a transgenic marker gene.
More recently, cell sorting techniques reliant on fluorescently tagged transgenic marker proteins have been employed to purify specific cell types and thereby refine the screen for potential regulators of gonadogenesis [21], [22].
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The two transgenic mouse lines were crossed, and double transgenic markers of Cre and LacZ were confirmed by PCR with genomic DNA isolated from their progenies.
However, inserting transgenic markers into established natural polyploids and/or production of advanced polyploid generations from synthetic lines will take a long time.
Since we are especially interested in their use as transgenic markers, and not as fusion protein candidates, tetramerization was not deemed to be a negative factor.
We do not know whether these similarities extend to fibroblasts, but live imaging in our zebrafish model of injury in combination with transgenic markers of fibroblasts might be able to address this question in the future.
In addition, the two markers in the diploid tester line 3A yield stable recombination rates over several generations in agreement with genetic maps established with non-transgenic markers.
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