Bjerknes et al. provided the evidence for the existence of multipotent stem cells in the adult mouse gastric epithelium using chemical mutagenesis to label random epithelial cells by loss of transgene function in transgenic mice (Bjerknes and Cheng 2002).
The results suggested that the expressed amiRNAs in the transgenic plants functioned as the small RNAs that determined the silencing of the gene(s) of interest.
Using transgenic models, functions for Myc - some potential, others data based - have been discussed for embryonic development, pregnancy, lactation and involution.
Unlike the above five genes, the functions of the other genes were identified through transgenic loss of function studies.
The potential of transgenic plants to function as efficient bioreactors for production of recombinant proteins has generated enthusiasm for this alternative production system.
To investigate the role of nuclear CaMKII in cardiac function, transgenic mice were designed and generated to target the expression of a CaMKII inhibitory peptide, AIP (KKALRRQEAVDAL), to the nucleus.
During HIF-1 expression in adult transgenic mice, ventricular function deteriorated and the heart dilated.
To test the hypothesis that specific amino acids in this region were necessary for Tre1 function, transgenic constructs were created where individual or small groups of amino acids in the deleted region were replaced with alanine.
To examine TaNAC29 function, transgenic Arabidopsis plants overexpressing TaNAC29 were generated.
Knockout, conditional knockout and gain of function (transgenic) approaches applied to study the role of Jun and Fos proteins during development and in diseases.
Therefore, for the future study of miR-125b function, transgenic miR-125b animal models will be needed which allow for specific overexpression/knockdown in individual cell types and tumor types that reflect better the actual situation in human cancer patients.
