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Plantlets were confirmed as transgenic by screening for the presence of the transgene and the selectable marker gene using PCR (Additional file 1: Figure S2).

In rice, decreased expression of OsCKX2 in transgenic by antisense also leads to an increase in grain number in the panicles (Ashikari et al. 2005).

One CP embryo was not transgenic by PCR genotyping.

This model withstands molecular level proof-of-principle using both a model cell line and primary embryonic thymic organ cultures rendered transgenic by lentiviral transgenesis.

Founder mice (20 days of age) were shown to be transgenic by PCR using tail tissue lysates and primer sets specific for: MMTV promoter (forward 5'-CTAAATTCAGAAGTTAGAAATGGG-3'; reverse 5'-CCAAAACTTACTTAAGCCCTGG-3'), human HER2 (forward 5'-GTGCACCGGCACAGACATGA-3'; reverse 5'-TTGTTCAGCGGGTCTCCATT-3') and luciferase (forward 5'-GGAAGACGCAAAAACATAAAGAAA-3'; reverse 5'-TGAGATGTGACGAACGTGTACAT-3').

The hairy roots identified as being transgenic by GUS staining and GFP detection were transferred to RM for shoot induction.

Cloning of transgenic BY-2 cells: generation of a cell line with strongly reduced heterogeneity.

Transgenic BY-2 cells were produced by co-cultivation with A. tumefaciens as described [ 41].

Stable transgenic BY-2 cells expressing AtHaspin-GFP were observed using a fluorescence microscope (IX-81; Olympus).

To analyze the relationship between AtHaspin and microtubules, we observed transgenic BY-2 cells expressing GFP-α-tubulin and inducibly expressing AtHaspin-tdTomato.

Wild type and transgenic BY-2 cells were imaged using a Leica TCS-SP spectral confocal microscope equipped with an argon ion laser using a 40 × oil immersion Plan-Apo objective (Leica, Wetzlar, Germany).