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MARs can also increase the transgene expression level and buffer transgenic silencing when the expression vector is inserted [ 7– 11].
We have earlier described a P25-expressing line with very low transgene expression level, that caused no detectable phenotype alterations in the plant, but for this study we have chosen a T3-line that accumulates P25 mRNA on higher levels.
Using the transgenic line (TG9) with the highest transgene expression level we demonstrated that the reduced Xac susceptibility might be ascribed to accumulation of H2O2 which was, at least partially, produced by PAO-mediated polyamine catabolism.
For example, a number of housekeeping genes should be satisfactory controls for comparisons of transgene expression level in the same tissue type from different transgenic lines grown under identical conditions.
The transgene expression level was quantified using the ΔCt method for relative quantitation and expressed relative to the level of GAL4 transcript for each line.
However, hydrogels with HA maintained this transgene expression level for an additional 2 weeks before declining.
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However, care must be taken in future studies toward the development of a possible immune response against the reporter transgenic protein when high transgene expression levels are required.
Significantly, the mutant VAPB inclusions did not occur in mutant VAPB transgenic mice from line VM2, which showed lower transgene expression levels than the other lines.
It has been shown that transgene expression levels decrease with aging in vivo from transgenic pigs [ 12].
This suggests that adjustments in transgene expression levels had occurred during memory cell development.
We cover basic aspects of retroviral gene transfer, regarding transduction efficacy and transgene expression levels.
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