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Today's large organizations are transforming with a top-down approach that focuses on customer centricity, marketing technology, digital advertising, e-commerce and cross-functional team alignment.
Alleles were replaced in both the evolved and ancestor strains by transforming with a URA3 plasmid that contained a portion of the targeted gene with the desired mutation and was digested at a single cut site within the target gene (Rothstein, 1991).
The evolved clones already constitutively expressed YFP, and a constitutively expressed CFP version of each clone was made by deleting the P ACT1 -ymCitrine- URA3 construct from the URA3 locus and then transforming with a ura3Δ::P ACT1 -yCerulean- URA3 construct.
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Sometimes, researchers try to transform (with a logarithmic transformation) a Poisson distribution with many zeros into a normally distributed variable.
As controls, we used the original Desirée line used for transformation and one line transformed with a PB33 GUS construct [ 3].
Data were transformed with a natural logarithm transformation when required (i.e. symbiont concentration).
Data have been transformed with a square root transformation.
We evaluated cells transformed with a low-copy centromeric plasmid containing ABF2 CEN-ABF2+ CEN-ABF2+empty versus transformants (Cemptyctor).
MT2012-ptxD was transformed wash a pMW118-based transformed expression plasmid.
Since then the neighborhood has been completely transformed, with a binge of residential construction.
The business will be transformed with a £500m-investment and renamed Bunnings.
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