Sentence examples for transforming strains from inspiring English sources
Leakage of ssGFP and Spr1-GFP from the spore wall was assayed by transforming strains to be tested with high copy plasmids carrying SPR1-GFP or ssGFP and sporulating them in 2% KOAc at 30°C for 20 hr.
Strains PH1223 (devR P nfsA :: mCherry), PH1224 (fruA P nfsA :: mCherry) and PH1225 (exo P nfsA :: mCherry) were generated by transforming strains DK5279, DK11063, and PH1244, respectively, with pFM17, as above with oxytetracycline selection.
Strains were prepared by transforming strain IL05 with each lacI-gfpi plasmid.
The ΔBAR1 mutants were generated by transforming strain PH-1 as described below.
After transforming strain TM1stop carrying the PlevD-cat fusion, each mutant was cultured on glucose, fructose or mannose and assayed for CAT activity.
Strain PH1226 was generated by transforming strain DK5208 with pAL4, as above with kanamycin selection.
S. cerevisiae strain TMB3452 was constructed by transforming strain TMB3043 with YIpDR7, linearized with EcoRV.
All evidence indicates that variability levels in the transforming strain B95-8 arextremelylowlow.
Finally, strain PM87 was created by transforming strain SK112 with plasmids pPM5 and pMS164 to express FliCS219C and CheYD13K, respectively.
Only 279 positions were putatively polymorphic among the 61 LCLs, which were infected solely with the transforming strain.
The double mutant of P MET3-MMS22/P MET3-MRC1 was generated by transforming strain CaLY226 with mrc1∆:: creating (CaLY228g CaLY228), followed by ARG4-MET3p-MRC1 CaLY251e (CaLY251g CaLY251).
