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One enzymatic activity was defined as the amount of enzyme transforming 1 µmol DMP min−1 (Moreira et al. 1997).
One enzymatic activity unit was defined as the amount of enzyme transforming 1 µmol of veratryl alcohol per minute (De Jong et al. 1994).
For all enzymes determination, one activity unit was defined as the amount of enzyme transforming 1 μmol of substrate per minute.
One activity unit (U) was defined as the amount of enzyme transforming 1 μmol of ABTS per min. Methyl syringate (methyl 4-hydroxy-3,5-dimethoxybenzoate) from Alfa Aesar (Karlsruhe, Germany) was used as the mediator.
Instead, as was obvious from the chromatograms and the respective mass spectra, nucleophilic substitution of the chlorine atom in 1 by a hydroxyl group occurred, transforming 1 into to the derivative 6.
Transforming (1) results in: rkern0.5em =kern0.5em Rkern0.5em -kern0.5em frac{z}{ tan kern0.5em beta } (3).
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These results demonstrate that isomer 1-I is thermodynamically more stable than 1-II in PBS buffer, and the kinetics of transforming 1-I to 1-II is about 2-fold slower than that of transforming 1-II to 1-I.
1,3-Propanediol dehydrogenase (DhaT) and aldehyde dehydrogenase (AldD) from P. putida KT2442 are capable of transforming 1,4-butanediol (1,4-BD) to 4-hydroxybutyrate (4HB) (Zhang et al. 2009a).
Glycolysis was lumped to three fluxes transforming G6P into PYRcyt.
To do this we transform (1).
We transformed MB210 with ACC02.2 and we obtained 2.8×107 transformants.
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