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SH773 was transformed with this PCR fragment [ 30], and transformants were selected as Ura+ colonies.
Mouse fibroblasts transformed with this clone bind serotonin agonists and antagonists and exhibit an increase in intracellular Ca2+ concentrations in response to serotonin.
Cre-expressing cells were transformed with this plasmid and subsequently infected with a recipient lambda phage that carried a stuffer DNA segment flanked by lox Pwt and lox P511 sites.
The coding region of pk cDNA was subcloned in pKM260 expression vector in correct frame and the protein was expressed in Escherichia coli BL21 (DE3) transformed with this recombinant expression plasmid.
Electro-competent C58C1 Agrobacterium tumefaciens were transformed with this construct, which was then used for agroinfiltration of wild type Arabidopsis flower buds.
The product was cloned into pPROEX-1 expression vector in order to get pPROEX-1-CWP1 afterfthatthat DH5α E. coli was transformed with this construction.
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Although the total protein also increases in lines transformed with the silencing fragment 'go', this should be interpreted with caution, because the increase in total protein was associated with a decrease in thousand seed weight.
Escherichia coli DY330 cells [40] were co-transformed with this fragment and with pUC18 containing the cbbL-cbbS region of the H. neapolitanus cso operon to replace the wild type cbbL-cbbS region on the plasmid with that of the insert by homologous recombination.
Each mutated and WT alleles presented in the table was co-transformed with this rpsL allele that was used as a surrogate marker for the selection of transformants (see Methods).
The xylose phenotype in GSY4326 or GSY4327 transformed with a plasmid containing this construct is the same as GRE3 CBS1502 with its own promoter, indicating that changes in the promoter sequence are not responsible for the increased growth in xylose.
Ramsey, though, is a player transformed, with five goals already in this competition so far this season, including the crucial headed winner at Dortmund earlier this month.
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