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Caspase-2 reporter strain yeast were transformed with these libraries, then transformants were stained with Xgal.
Several plant species including Arabidopsis, tobacco, poplar, rice, Eastern cottonwood, peanut, salt marsh grass and Chlorella have been transformed with these genes.
A diploid strain heterozygous for deletion of CDC24 (RLY2775) was transformed with these fragments.
Then, yeast cells were transformed with these plasmids and grown on SC-URA-LEU media.
B. neotomae was transformed with these plasmids and the expression of β-galactosidase in the recombinant bacteria was measured.
We also analyzed the palmitoylation status of Tlg1 in sw1fΔ strain transformed with these SDP mutants, using the biotinylation assay.
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The adherence of the ligA transformant was elevated by 55±14% (seven independent determinations) compared to either the native wild-type, nonpathogenic strain or these cells transformed with the empty pSLe94 vector.
The uptake efficiency of both galactose and glucose was compared in S. cerevisiae hxt null mutants transformed with each of these six genes (Additional file 3A).
Then, A. tumefaciens cells transformed with each of these constructs and p19 protein of tomato bushy stunt virus were infiltrated into leaves of Nicotiana benthamiana.
An Agrobacterium tumefaciens suspension culture transformed with each of these BnaCBL- or BnaCIPK-GFP constructs, together with the corresponding marker construct, was co-infiltrated into leaves of N. benthamiana, and the GFP and mCherry signals were examined two days later.
Nematodes transformed with either of these fusion genes only showed weak pharyngeal reporter expression, which has been thought to be a common artifactual reporter expression domain (see above) and is in contrast to the strong, specific reporter expression driven by the ceh-33 and ceh-34 Promoterome inserts.
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