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Furthermore, some of the differently expressed genes were also modified when cells were transformed with specific tsRNAs contained in EVs.
E. coli cells transformed with specific dnaQ mutant were spread on LB agar plates and cultivated overnight in 37°C.
Likewise, MSC transformed with specific mutations play an important role in sarcoma genesis and in the control of sarcoma tumor growth [ 136, 137].
Subsequently, we assessed NRP2 expression in normal mammary epithelial cells that had been transformed with specific oncogenes: HMEC transformed with large T antigen, hTERT and Ras (HMLE-PR) and MCF10A cells transformed with T24 H-ras (MCF10A-AT).
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First, wideband chirp signal was transformed with a specific transform order into sine wave with a single frequency in fractional Fourier transform (FRFT) domain, so that the time-invariant steering vector of the wideband chirp can be obtained.
AAP could be transformed with a maximum specific degradation rate, half-saturation constant and inhibit constant of 1.775/36 h, 175.3 mg L−1, and 396.5 mg L−1, respectively, which proved that the degradation rate of AAP could be restrained at high AAP concentration.
Thus, this study provided functional confirmation that ependymoma variants indeed arise from their matched populations of RGCs transformed with the subgroup-specific mutations.
The transient gene expression assay [ 6, 11, 12, 18] is based on transformation of single leaf epidermal cells, followed by subsequent inoculation of transformed leaves with specific powdery mildew isolates.
We tested the activation of PI 3-kinase in THP-1 cells stably transformed with a reporter construct specific for the kinase product phosphatidylinositol (3,4,5) triphosphate (PIP3) [53].
To determine whether the T2245C mutation observed was responsible for clarithromycin resistance, the 26695 claS H. pylori strain was transformed with genomic DNA and specific PCR products.
MCSs between ER and plasma membranes have not been reported for plants, but a close proximity between these membranes has been visualized by freeze fracture microscopy of suspension-cultured sycamore cells [ 13] and by confocal microscopy of Arabidopsis transformed with fluorescent tags on specific ER membrane proteins [ 14].
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