Ramsey, though, is a player transformed, with five goals already in this competition so far this season, including the crucial headed winner at Dortmund earlier this month.
PCR analysis indicated that about 30% of the transgenic plants were transformed with two different T-DNAs and additional 9.5% of the plants carried all genes from three different T-DNAs.
The in vivo activity of the CBD IB-based metabolon was examined through the determination of 1-butanol synthesis using E. coli transformed with two plasmids containing the LZ-fused CBD and LZ-fused 1-butanol pathway genes, respectively.
In order to obtain substantial quantities of active papain, the DNA coding for propapain, the papain precursor, has been cloned and expressed at a high level in Escherichia coli BL21 DE3) transformed with two T7 promoter based pET expression vectors – pET30 Ek/LIC and pET28a+ each containing the propapain gene.
S. coelicolor M145 protoplasts were transformed with one of pSET152, pSET152ssrA or pSET152ssrA-DD and simultaneously with pGM160ssrA::Hyg.
To test for recombination, an E. coli strain containing a substrate was transformed with two compatible plasmids, each expressing one ZFR variant.
Yeast transformations were performed according to the LiAc/PEG/ss-DNA protocol [32] and both strains were transformed with one or more of the constructs described below, in parallel with the corresponding empty plasmid(s).
Strains with disrupted non-essential HAT genes (Δgcn5, Δhat1, Δsas2, Δsas3 and Δrtt109) or mutations in the essential ESA1 gene esa1-4144, esa1L245P [27]) were transformed with two different mini-chromosomes carrying ARS1 (pARS1wt) or ARS305 (pRS315).
Control experiments including mock transformations with empty plasmid vector alone or omission of anti-myc IgGs during immunofluorescence staining of BY-2 cells transformed with myc-OEP9 yield no (epi fluorescence, as expected (Figure S2a).
