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E. coli transformed with appropriate plasmid vectors express human β1 and β2-adrenergic receptors that retain the binding properties of the corresponding receptors in mammalian tissues.
Just before her guests arrived, she picked up her entree, which now rested on a pretty (restaurant) platter, lovingly transformed with appropriate vegetables and spices into roasted Greek chicken.
30 ODs of swf1Δ strains transformed with appropriate plasmids were harvested by centrifugation and resuspended in 300 µl lysis buffer (PBS, EDTA 2mM, PMSF 1mM and protease inhibitor cocktail (PIC).
All the anti-T1, T2 and LD1, LD2 antisera were adsorbed against lysates of bacterial cells transformed with appropriate pET expression vector expressing the GST or His tag to remove anti-GST or - His antibodies [38].
The blots were probed using secondary antibodies coupled to either IRdye 680 or IRdye 800 (LICOR bioscience, UK) at 1/20000 dilution, and then scanned using an Odyssey Infrared imager (LICOR bioscience, UK). 30 ODs of yeast strains transformed with appropriate plasmids were spheroplasted with zymolyase.
Prominent amongst existing metazoan systems for efficient recombinant protein expression are those utilizing cell cultures derived from lepidopteran insect cells, either as hosts for baculovirus expression vectors [16] or as cell lines stably transformed with appropriate plasmid-based expression constructs [17].
Similar(49)
First, the problem is transformed via the Laplace transform (with appropriate initial conditions) into a reduced wave equation.
These proteins were expressed in E. coli BL21 (DE3) pLysS cells by transforming with appropriate plasmid pET15b constructs.
Proteins were expressed in BL21 (DE3) pLysS [F− ompT hsdSB (rB− m B−) gal dcm (DE3) pLysS (cam R)] cells by transforming with appropriate plasmid constructs.
Briefly, E. coli (BL21 DE3) were transformed with the appropriate plasmid and grown in 1 l of LB media with the appropriate antibiotic.
The yeast cells transformed with the appropriate Rho expression plasmid were pre-grown overnight under repressing conditions with 5 μg/ml Doxy at 25 °C in a shaker (255 rpm).
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