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TEFp-xylB-TEFt was amplified from pAGS7 with primers 308/309 containing 50 bp flank to the GRE3 locus and transformed together with gRNA.GRE3-natMX (LWA25) into CEN.PK 113-7D already containing Cas9-kanMX.
Finally, the data were z-transformed within participants; for participants performing both CRT and WM tasks (Experiment Two), both tasks were transformed together to retain task differences.
The recombinant plasmid was transformed together with the vector pREP4 (Qiagen) into Escherichia coli BL21 (DE3).
Plasmids expressing ILV2ΔN54, ILV5ΔN48 and ILV3ΔN19 were transformed together into this strain.
The three fragments were subsequently transformed together with XhoI linearized pRSnat (Klix et al. 2010) into yeast strain PJ69-4A.
Bait and prey vectors were transformed together in the MaV203 yeast strain using the ProQuest™ Two-hybrid System (Invitrogen), according to the manufacturer's manual.
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pSP-GM1 was linearized with restriction enzyme BglII (ThermoFisher), purified, and co-transformed together with purified xfpk PCR-fusion constructs into CEN.PK 113-5D, and transformants were obtained by growth on SD-URA plates.
Building on the ambitions of pioneers and vanguards, we can better meet the needs of patients if we transform together – a point made forcefully by Simon Stevens at the Kings Fund recently.
The Parseval formula for the Fourier transform together with inequality (2.18), there holds (2.34).
Cities change and mutate, they transform together with the civilisations living in them.
"You can't separate markets anymore by service provider, Internet, wireless, cable, home they will all transform together," he says.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com