Sentence examples for transformed subsequently from inspiring English sources

The expression hosts (E. coli BL21-Gold (DE3) or B. subtilis DB104) were transformed subsequently with the isolated plasmids, Step IV: agar plate pre-screening with selection based on halo formation, and Step V: screening in microtiter plate format to quantify increase in enzyme production.

However, where binary outcome measures are required, results based on continuous measures can be transformed subsequently using cut-off points, [ 49].

For each tumour, individual voxel time-courses were Fourier transformed and subsequently combined into a single average-frequency spectrum.

Each dataset was first log transformed and subsequently z-score normalized within each sample within each dataset in order to minimize batch specific effects.

To test this possibility, a plasmid rescue experiment was performed and the plasmid DNA extracts from the pHL015-transformed X514 were subsequently transformed into E. coli.

After the excision of theURA3 gene, the transformant with split chromosome IV was subsequently transformed with the same vector.

Second, Jacobian scaled ("modulated") images were calculated and subsequently transformed to Montreal Neurological Institute space using affine transformation.

This disruption plasmid was propagated in the methylation deficient E. coli host ET12567 and subsequently transformed into S. coelicolor M145 through protoplast transformation.

YFP-AtBS14b fusion protein was subsequently transformed into N.benthamiana leaves by using Agrobacterium-mediated transformation.

The yielded fragments were subsequently transformed into pCR4Blunt-TOPO vector (Invitrogen, Paisley, UK) and after restriction digest ligated into pGL42a.

Considerable private construction subsequently transformed much of central Boston.