Sentence examples for transformed sequentially from inspiring English sources

Heat-induced DY380 cells containing the retrieved DNA segment were transformed sequentially with MTVs which had been excised from the pBluescript vector backbone to generate the final targeting vector.

ROS generation appears to be triggered by the activation of the mitochondrial-dependent cell death pathway through the proapoptotic Bcl-2 proteins Bax and is further transformed sequentially into more toxic ROS, like hydrogen peroxide, which, consequently, induces cell death [ 44].

The strains were made prototrophic by transforming sequentially the CEN.PK2-1C strain with (i) the required expression cassettes for LEU2, TRP1 or HIS3, (ii) the GPD1 and/or GPD2 deletion cassette and (iii) YEpJK01plasmid.

A kernel principal component analysis (KPCA) model, which not only captures nonlinear relationships between variables but also reduces the dimensionality of the data, is built with the reconstructed data obtained by performing wavelet transform and inverse wavelet transform sequentially on measured data.

To uncouple this cycle of response and counter response between plant and insect attackers, we fed larvae on isogenic plants that had been transformed to sequentially silence a suite of JA-mediated defenses (N, TPIs, and all JA-mediated defenses) and analyzed the transcriptional responses in neonates with an unbiased experimental protocol.

These two plasmids were sequentially transformed into E. coli, generating a library of ∼5 × 10 transformants.

It explains exact linearization of used, initially nonlinear models, which are sequentially transformed to discrete state-space form.

The yeast reporter strain AH109 was sequentially transformed with pGBKT7-Dmknδ5 and a cDNA library (Matchmaker human keratinocyte library in pGAD10, Clontech) following the instructions of the Matchmaker Gal4 two-hybrid system (Clontech).

Yeast were sequentially transformed with bait plasmid and pACT2 library by the lithium acetate method.

The Sep-OTS plasmid and split fluorescent protein duets were sequentially transformed into electrocompetent E. coli by electroporation.

Then, the plasmids were sequentially transformed into yeast strain AH109 through the lithium acetate method following the protocol described in Yeast Protocols Handbook (Clontech).