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The plasmids of pYES2- SbMYB44 and vector alone were transformed separately in yeast strain W303 using Yeastmaker yeast transformation system (Clontech).
The wavelet transform (WT -based JPEG 2000 is a standard for the compression of digital images that uses a separable lifting structure in WT -basedultidimensional image signal is transformed separately along its horizontal and vertical dimensions.
The 35S BrGRF8 and vector control constructs were transformed separately into Agrobacterium tumefaciens LBA4404 and then the A. tumefaciens-mediated transformation of Arabidopsis was performed via vacuum infiltration [ 30].
Each feature vector was transformed separately into a smaller dimensional subspace using LPP before classifying the concatenated final feature vector using a SVM with an RBF kernel.
Contributions of specific reactions to the mass balances are identified and transformed separately, so that a modular approach is possible for the construction of the pgf balance equations for different free radical processes.
The resultant pMD19T-C-Z was digested with Bgl II and Sal I, and the 2100 bp fragment C-Z was transformed separately into P. pastoris GS115 and ZJGSU1.
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Then, the coefficients will be processed by wavelet transform separately and four different frequency components of the coefficients can be obtained (shown in Fig. 1).
Before and after this training phase, the network was presented with each of the novel stimuli individually translating across the input layer in order to test and record the output layer neurons' responses to each transform separately.
For each cell in the ensemble vector, its firing rate response to each unknown transform is separately fitted to a Gaussian distribution of firing rates to each stimulus.
These four plant transformation vectors were separately transformed in to Agrobacterium.
The pTrcHis plasmids carrying each rSsp4 mutant were separately transformed into E. coli, while the pJIR751 plasmids carrying each mutant ssp4 gene were separately transformed into a previously-created SM101 ssp4 null mutant (SM101::ssp4) [15].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com