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In the presence of high salt, vector transformed seeds showed almost no germination (only one out of thirty-two seeds in one repeat) until 20 d, while on average 85 95% CaZF overexpressing T1 seeds showed germination within 15 d (Figure 4C).
On average, the proportion of C18-PUFAs increased from 43.1 ± 0.57% in empty vector-transformed seeds to 50.2 ± 0.26% and 51.6 ± 0.61% in LuPDCT1 and LuPDCT2 transformed seeds, respectively.
The proportion of transformed seeds was positively correlated with the amount of transformed pollen applied to stigmas.
However, under the same experimental conditions, both wild-type and empty vector transformed seeds failed to germinate.
To examine α-AI1 protein expression in coffee beans of T0 plants, crude extracts from transformed seeds were analysed using SDS-PAGE and immunoblots.
The ALA content in the metabolically transformed seeds was reduced in contrast to the oil from wild-type camelina (WCO) as a result of conversion to EPA (Ruiz-Lopez et al., 2014).
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Percent viability was excellent (99-100%) in both transformed seed samples and untransformed control.
Arabidopsis thaliana was transformed using Agrobacterium by floral dipping [ 56], and transformed seed selected on kanamycin.
The transformed seed did not accumulate NO under hypoxic stress and had higher respiratory activity and energy charge than the wild type.
A: untransformed and transformed rice seeds; B: untransformed and transformed tobacco seeds.
For a long time we have understood that for solid tumors, a single transformed cell seeds the growth, and that present-day tumor cells are descendants from a clonal expansion of the first malignant cell [1].
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