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When a stable morphological appearance was achieved, there was good correlation between transformed morphology and colony formation in soft agar.
Moreover, co-expression of a dominant negative form of p53, p53DD [42] and H-RasV12 by retroviral vectors in FHL2−/− cells resulted in thin, elongated, and spindle-like transformed morphology (see Fig. 3D).
In vitro studies showed that it had weak antibiotic properties and that it was able to revert the transformed morphology of an H-ras transformed cell to normal.
In most cases the clones underwent cell death, and the surviving population lost the transformed morphology and TRK-T3 oncogene expression.
In contrast, the TM-shShc cells maintained the transformed morphology, and even adopted a slightly more elongated and spindle-shaped appearance than the control TM-shCTRL cells.
Other clones maintained the transformed morphology and oncogene expression either because the ShcY317F protein was not sufficient to titer out the wild type or because they compensated the inhibitory effect of ShcY317F by accessory mechanisms.
Similar(48)
Prolonged annealing of the film transformed its morphology into inverted-spherical domains of PEO in the PFOMA continuous phase through long-range ordering by following the fission process.
The follow-up acidic treatment of the anodized WO3 films in H2SO4 solution transformed the morphology into plate-like WO3·2H2O.
Reduction of Stat3 in NIH3T3 foci is related to increased microtubules destabilization, as indicated by reduced acetylated α-tubulin and transformed cell morphology.
On the other hand, exposure to RA also transformed cell morphology towards an elongated appearance.
In our study, data demonstrated that MTDH overexpression transformed the morphology of NSCLC cells from metastatic slender, spindle-shaped mesenchymal-like shape to polylateral epithelial-like structure.
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