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Each transformed line, derived from a single transformation event, has been multiplied in vitro for over two years, and several different harvests have been tested spectrophotometrically and via HPLC, finding only minor variations in carotenoid content (Table 3).
In contrast to tissue subcultured from wild-type and mutant lines, the positive and negative control transformations produced multiple colonies, each representing an independent stably transformed line, that could be directly and quantitatively compared to colonies derived from test transformations.
Based on transformation efficiency, survival rate, and assuming only 10% of transgenics are germline transformants, Li et al. (2011) estimated that generation of a single germline transformed line would require 167 transgenic iL3s in S. stercoralis and 20 transgenic iL3s in S. ratti.
The original culture possessed a normal human male karyotype, and the transformed line was largely hypotetraploid.
Post-embryonic development of beetles fed with the foliage of the nptII-gus transformed line exhibiting the higher β-glucuronidase activity is shortened by more than 2 days.
The induced excision events and efficiency in each transformed line were determined by presence of the 450-bp and/or 550-bp fragment (Figure 3).
Similar(34)
When ppcesa5KO-2 was transformed with the empty negative control vector, only 13% ± 4.9% of lines (average for seven independent transformations: 182 stably transformed lines) produced gametophores.
Lignin content was reduced in transformed lines compared with their non-transformed counterpart (P<0.05).
This result confirms that the transformed lines correspond to two independent transformation events.
The evaluation of several independently transformed lines is part of tests for side-effects of the transformation procedure.
R1, R5 and R10 are independently transformed lines.
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