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The mutant strain was transformed further with the pCP20 plasmid to cure the Kan and produce a Kan-sensitive mutant strain that was named BL21∆luxS.
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In both ovary and testis, progesterone is transformed further to the androgenic steroid androstenedione (17c), which, together with its derivative testosterone (17e), is secreted by the testis.
Working with Ms Morris and Margaret Hodge I am determined not to miss this once-in-a-generation opportunity to transform further education.
Wind energy costs are falling more slowly, but this could be transformed with further innovation, the report says.
Continuous variables were tested for normality with the Kolmogorov-Smirnov test, and if P < 0.05, they were logarithmically transformed for further analysis with the help of generalized estimating equations (GEEs).
These pTUM4 transformants were further transformed with pASK-IBA32 plasmid encoding the Bj-xtrIT(His6) gene and plated on agar with 100 μg/ml AMP plus 25 μg/ml CAM overnight at 37 °C.
To obtain tetracycline-regulatable expression of GFP TbAGO1 in the knock-out background, this cell line was further transformed with plasmid pHD360 (linearised with Not I), that expresses the tet-repressor after integration in the tubulin locus [ 34].
The self-adjointness and the orthogonality condition for the eigenfunctions of the further transformed system with elastic boundary conditions are derived.
Some derivatives were further transformed either with the simian virus 40 (SV40) large T antigen, termed HEK293T, or with the Epstein Barr virus (EBV) nuclear antigen 1 (EBNA1), termed HEK293E, in order to mediate semi-stable episomal propagation of vectors containing an origin of replication (ori) of SV40 or EBV, respectively.
Strain KA418 (Δ rseP /Δ ompA /Δ ompC )/pEVOL-pBpF was further transformed with a plasmid encoding an RseP-derivative with an amber mutation at the indicated positions.
Transformation of A. thaliana with the first two genes consolidated on a single binary vector yielded transformants producing high levels of DGLA, and these plants were further transformed with mouse prostaglandin H synthase (PGH) genes to produce prostaglandins.
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