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Growth inhibition responses were not transformed for testing in the GLM.
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Variables not normally distributed were log-transformed for testing; descriptive summary statistics and plots are presented using the original scale.
A normal distribution of variables was determined by the Kolmogorov-Smirnov test and serum ferritin values were log transformed for statistical testing.
Data were converted to relative quantiles and log2 transformed for statistical testing.
Nonnormally distributed variables (triglycerides, apoB, IL-6, and CRP) were log transformed for statistical testing, and differences in geometric means are reported to present these variables in their original scale.
For quantification, n = 3 biological replicates, error bars represent ±1se, *p<0.05, ***p<0.01 by Student's t test, data were log transformed for t test to avoid issues caused by the very large difference in dsRNA quantification between wt and RNAi+ cells, y axes in arbitrary units.
Viremia titers were log transformed for statistical tests.
Slope values were log transformed for statistical tests because of their skewed distribution.
All values of Δ R S C U ― + were log transformed for these statistical tests so they fitted the normal distribution.
This alone will make it a very useful model for understanding how cells that have lost Brca1 become transformed, and for testing treatments aimed at blocking that process.
Recall that data were log transformed for ANCOVA and t-test analysis to address skewness and kurtosis and ensure the requirements of the statistical tests were met.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com