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Therefore transformed files were manually reviewed.

The calculated six element transformation matrix is output to the transform file (outfile).

It is possible to input values for these four parameters directly (deltax, deltay, rotang, scale), in which case the transform file must be left blank.

The asol file aspect solution or the FITS image or table file WCS tangent point should be in the same general region of the sky as the tangent point of the WCS from the transform file.

The first step of our method transforms files into a more abstract representation, their logical structure.

The AGCC software (Affymetrix GeneChip Command Console) transformed DAT files into CEL files to change image signals into digital signals, which recorded the fluorescence density of probes.

Ct values for all reactions were log2 transformed (Additional file 2: Table S3 and S4).

After intensity analyses, the log2 transformed signal file was visualized by the integrated genome browser (Affymetrix).

For further analysis, TPM values were log2 transformed (Additional file 14).

Adjacent mammary gland whole mounts from tumour-bearing mice were also fully transformed (Additional file 3: Figure S3A).

In fact, the  .csv files can be easily transformed into.nirs files.

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