Exact(2)
The transformed expressions were then clustered using the fuzzy c-means (FCM) clustering algorithm in the Bioconductor Mfuzz package [ 89].
Taking MPSS 17-bp signature data as an example, the Pearson correlations of the logarithmic transformed expressions between MPSS data and microarrays ranged from 0.39 (MPSS – Illumina) ~0.48 (MPSS – Affymetrix) for MRP1, and 0.40 (MPSS – Illumina) ~0.52 (MPSS – Affymetrix) for MRP2.
Similar(58)
We applied qtscore function from the GenABEL package to quantile transformed expression values for genes expressed in more than 90% accessions and binarized expression values for genes expressed in between 50 and 126 accessions.
Differentially expressed genes were determined by fold change (fc) calculation of log2 transformed expression data.
(3) Perform PCA-based dimension reduction using the z-score transformed expression of highly variable genes.
CEL-files were imported into Partek Genomic Suite v6.6 software (Partek Inc., St Louis, MO) for performing robust multichip average normalization including background correction, quantile normalization across all arrays, and median polished summarization based on log transformed expression values.
Log 2 transformed expression values were annotated with HUGO symbols.
Logarithmically transformed expression values were used to implement GSEA.
Next quantile normalization was applied to the log2 transformed expression values (Bolstad BM Bioinformatics 19: 195–193).
The transformed expression data, corresponding to 66 ratios, were then used for Fisher's Exact Test.
Figure 4E represents scatter plots of the natural log transformed expression values of all genes (22690) on the array.
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