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Kolmogorov-Smirnov normality test (Lilliefor's test) was first applied to the quantification data gathered from the 24 independent transformants that were regenerated per transformed construct (96 in total).
This minimizes the possibility of selection for insects resistant to the transformed construct.
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These transforms construct a general representation of signals which can be used in detection, diagnosis and treatment monitoring.
In contrast, in the three or four dropout plates, no growth was observed for the pairs of transforming constructs pGBK-VP51A/pGADT7 and pGBKT7/pGAD-VP19, or for the negative control (Figure 1B, panels b and c).
(We also note that we had difficulty transforming constructs containing H. influenzae operators into E. coli, although constructs from the other species could be readily transformed).
Transfection of cloned plasmid DNA into benign tumor cells has revealed that transforming constructs of the fos oncogene induce malignant conversion, whereas myc and adenovirus E1A oncogenes do not.
We subsequently transiently transformed the construct into tobacco leaves, and visualized the CFP signal under a confocal microscope (Figure 4D).
FBAT P-values were −log10 transformed to construct Figure 3.
E. coli BL21 (DE3) was transformed with construct pET28a-PRSS1dAP.
We transformed the construct into N. tabacum using the method described by Horsch et al. [ 59].
For higher resolutions, the algorithm relies on a secret transform domain constructed with anisotropic wavelet packets (AWPs).
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