Sentence examples for transformed and maintained from inspiring English sources

The influence of 19th- and 20th-century missionaries has both transformed and maintained aboriginal integration.

The Paulinskill Valley Trail a network of rail trails along abandoned railroad beds of the New York, Susquehanna and Western Railroad have been transformed and maintained for hiking, horseback riding, and other recreational uses, stretches for 27 mile from Sparta Junction in Sussex County to Columbia in Warren County, roughly following the entire length of the river.

Following hatching, the sporocysts were transformed and maintained in sporocyst medium supplemented with ∼20 µg/ml of dsRNA.

The only vector employing positive selection by kanamycin has a backbone from the plasmid pRSF (Merck Biosciences), is streptomycin resistant and can be transformed and maintained in an E.coli strain which simultaneously harbors a second plasmid with a colE1 origin like the pET-derivatives using chloramphenicol or the pQE80 derivative using ampicillin for selection.

The recombinant plasmids were transformed by electroporation and maintained in JM109 cells.

They were either used directly or transformed to promastigotes and maintained until the second to fourth passage cultures before the promastigotes were used.

Transformation by microinjection was as previously described [ 10] with pRF4 and the plasmid containing the GFP fusion injected as a mixture, both at 100 μg/ml, and transformed lines established and maintained by screening for individuals with the roller phenotype.

Cre recombinase-expressing cells were then transformed with pLox66 and maintained on solid EMM medium at 30°C for 24 h, followed by replica plating these cells to YES + G418 (200 mg/l) to select for cells with stably integrated pLox66.

The pGBKT7-Gal4-BD-PfPP1 construct was used to transform Y187 strain and maintained on SD media without tryptophan (SD-W).

Labor scarcity and land abandonment caused by rural labor out-migration have complex effects on land quality and soil erosion, especially in hill-mountain areas that have been substantially transformed by human settlement and maintained with labor-intensive production practices.

The transformed roots maintained in SH and B5 media with full and half-strength content of macro- and micronutrients were thick with an average root diameter of 0.9 1.5 mm and had short and small branches.