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Box Cox transformations were considered for the biomarker measurements and log transformations were selected for the regression analyses.
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After transformation, transformants were selected on agar containing tetracycline (12.5 μg/ml).
To address these questions, alchemical transformations are selected based on the thermodynamic cycle shown in Figure 4.
The resulting plasmid was transformed into S. pneumoniae R6 as described above (without the need for rpsL co-transformation) and transformants were selected on CAT agar supplemented with 600μg/mL kanamycin.
To identify infections by multiple variants, five clones from each transformation were selected for sequencing analysis.
In order to compare expression levels of the PvUbi1 and PvUbi2 promoters with those of other promoters, several plant promoters commonly used in monocot transformation were selected and cloned into the same identical vector (pHLucGWgus) to eliminate any discrepancies in expression levels as a result of vector backbone or vector size (Additional file 1, Figure S2).
For each transformation, 50 positive transformants were selected by tributyrin flat screening.
Hypocotyl segments of Z35 were used as explants for the transformation, and the transformants were selected using the method described by Sunilkumar and Rathore (2001) [ 62].
While transformants were found after transformation with DNA and PCR products from the BZ586 claR strain, none of the transformants were selected after transformation with DNA from the claS strain 26695 or TE.
The recombinant vectors pMADDelLicM1AC and pMADDelLicM2AC were then introduced in B. licheniformis MW3 via protoplast transformation [29] and transformants were selected at 30°C.
Following transformation of yeast strain Jo55, transformants were selected on galactose-containing media and grown to an optical density (OD600) of 1.0.
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